Insertion junction: LMJ.RY0402.235230_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.235230
Chromosome:chromosome 6
Location:5117662
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g281100 MUT9,ROC94 (1 of 2) PTHR11909:SF146 - CASEIN KINASE-LIKE PROTEIN; Serine/threonine protein kinase, transcription repression 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACGATAACACAGCGCACTCTGGCTGTG
Internal bar code:ACAACGCGTGTGTTAAAGGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:280
LEAP-Seq percent confirming:81.3017
LEAP-Seq n confirming:787
LEAP-Seq n nonconfirming:181
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTTCATGTGCCACTGTC
Suggested primer 2:AAGAAAAACACCCATGTCGC