| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235231 |
| Chromosome: | chromosome 1 |
| Location: | 936262 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTTGTAACGTCGCGTAGATATGTCAGA |
| Internal bar code: | TCGGGCCGTAGAGGACGCATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1156 |
| LEAP-Seq percent confirming: | 99.5057 |
| LEAP-Seq n confirming: | 28585 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGTGATTGATGGCAAG |
| Suggested primer 2: | GCCTACTAAGCACACGCCTC |