| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235231 |
| Chromosome: | chromosome 1 |
| Location: | 936265 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAGCGCATGTGGAGACCTCCAGGCTGA |
| Internal bar code: | AAAGGCCTCAGGGCGAGAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 98.9555 |
| LEAP-Seq n confirming: | 6158 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGTGATTGATGGCAAG |
| Suggested primer 2: | GCCTACTAAGCACACGCCTC |