| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235249 |
| Chromosome: | chromosome 16 |
| Location: | 1316930 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651650 | TSI3 | Isoleucyl-tRNA synthetase; (1 of 3) 6.1.1.5 - Isoleucine--tRNA ligase / Isoleucyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTACAAGTGACTGCCTTCAGAGCCTCC |
| Internal bar code: | CAGGGTCGGGGCTACAAGTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 97.6431 |
| LEAP-Seq n confirming: | 1160 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGCGAGGCTACAAGAAG |
| Suggested primer 2: | GCGGGCTGACCATAGTGTAT |