| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235249 |
| Chromosome: | chromosome 16 |
| Location: | 1316933 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651650 | TSI3 | Isoleucyl-tRNA synthetase; (1 of 3) 6.1.1.5 - Isoleucine--tRNA ligase / Isoleucyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGAGTACAAACGACCACCTTCTGTACCA |
| Internal bar code: | TGGATTTCCGAGCGTTGGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1220 |
| LEAP-Seq percent confirming: | 99.4024 |
| LEAP-Seq n confirming: | 5156 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGCGAGGCTACAAGAAG |
| Suggested primer 2: | GCGGGCTGACCATAGTGTAT |