Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.235259 |
Chromosome: | chromosome 13 |
Location: | 142968 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g563006 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTACAACCCCCCTGCCCCCCCCTCACACA |
Internal bar code: | TTCGAGTCTGGTTGTCCCCTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 257 |
LEAP-Seq percent confirming: | 86.8742 |
LEAP-Seq n confirming: | 642 |
LEAP-Seq n nonconfirming: | 97 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCATCCCAGTTCAGTACGC |
Suggested primer 2: | AGCTATGGGAGACCAACCAC |