Insertion junction: LMJ.RY0402.235259_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.235259
Chromosome:chromosome 13
Location:142968
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g563006 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTACAACCCCCCTGCCCCCCCCTCACACA
Internal bar code:TTCGAGTCTGGTTGTCCCCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:257
LEAP-Seq percent confirming:86.8742
LEAP-Seq n confirming:642
LEAP-Seq n nonconfirming:97
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:ACCATCCCAGTTCAGTACGC
Suggested primer 2:AGCTATGGGAGACCAACCAC