| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235276 |
| Chromosome: | chromosome 7 |
| Location: | 2131803 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g326500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCTCCTGGTGCCCCGACGCACACACGC |
| Internal bar code: | GGCCCGTAGTTTGGGCACTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 52.1883 |
| LEAP-Seq n confirming: | 5032 |
| LEAP-Seq n nonconfirming: | 4610 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCCCTACAAGGTCAACG |
| Suggested primer 2: | CTTCTAATACAGCGCGAGGG |