| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235278 |
| Chromosome: | chromosome 13 |
| Location: | 3323028 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586600 | (1 of 1) K08360 - cytochrome b-561 (CYB561) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGGATGGCACTGCAGGGGTGGCAGGAG |
| Internal bar code: | TCGCGACTACACTTGCGAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 99.1166 |
| LEAP-Seq n confirming: | 561 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGACTACAGCAACTGCCA |
| Suggested primer 2: | CACCTGGTTGCTTTGCCTAT |