| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235279 |
| Chromosome: | chromosome_3 |
| Location: | 4211974 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g174050 | MRP4 | ABC transporter, multidrug resistance associated protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACATAGGTGGGAGGTTCGTGAGGTGGTGA |
| Internal bar code: | AAACAACAGCGAGAAATGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 77.0704 |
| LEAP-Seq n confirming: | 2615 |
| LEAP-Seq n nonconfirming: | 778 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTGTGTGTGTGTGGGAG |
| Suggested primer 2: | CCTGAGCTTCGAGGTAGGTG |