| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235304 |
| Chromosome: | chromosome 6 |
| Location: | 3485707 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278099 | (1 of 2) IPR003382 - Flavoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCACCGTTCAGAATAACAGCGTTGCGT |
| Internal bar code: | TAGTTGTAATTACAGCAGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 62 |
| LEAP-Seq percent confirming: | 73.444 |
| LEAP-Seq n confirming: | 177 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACCTTCTGGACAGCGAG |
| Suggested primer 2: | GCAGAGCATGTTTGGACTGA |