| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235318 |
| Chromosome: | chromosome 3 |
| Location: | 293960 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144564 | MMP13 | Matrix metalloproteinase; (1 of 1) IPR008752//IPR024079 - Peptidase M11, gametolysin // Metallopeptidase, catalytic domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACCCTCTTTATCCGCCTACCCTTGCGC |
| Internal bar code: | TAGATGTAAGTGAACGGCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 378 |
| LEAP-Seq percent confirming: | 98.9818 |
| LEAP-Seq n confirming: | 1361 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAGCCCAAACTAAACCG |
| Suggested primer 2: | CACAACCAGTCAGCCAGAGA |