Insertion junction: LMJ.RY0402.235319_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.235319
Chromosome:chromosome 4
Location:1206983
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g215400 AKC3 conserved expressed protein related to ABC1/COQ8 mitochondrial putative ser/thr kinase; (1 of 2) PTHR10566//PTHR10566:SF86 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // ABC1 PROTEIN KINASE 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCATACCATTTGCCCATTCTCTCCCCAC
Internal bar code:CGTCACAGGCGGAGCTACTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:568
LEAP-Seq percent confirming:94.5936
LEAP-Seq n confirming:7926
LEAP-Seq n nonconfirming:453
LEAP-Seq n unique pos:110

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCATTTTCTCGCCAGAGG
Suggested primer 2:CACCATCTCACACACAAGGG