| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235325 |
| Chromosome: | chromosome 12 |
| Location: | 2109184 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509150 | (1 of 1) PTHR18937//PTHR18937:SF151 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTCAGGGCGGTCGGCTGGTAGTTAGCT |
| Internal bar code: | GTCTCACGACTAAGCAACGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1207 |
| LEAP-Seq percent confirming: | 99.3023 |
| LEAP-Seq n confirming: | 3416 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCTTTATTAAGCGGGCG |
| Suggested primer 2: | ATGCTGACAACAACAGGCAG |