| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235368 |
| Chromosome: | chromosome 17 |
| Location: | 5307329 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736300 | (1 of 1) IPR000104//IPR011047//IPR018391 - Antifreeze protein, type I // Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCTCGGGCATGTAACACCCCTCCTCCC |
| Internal bar code: | TCGCGTAGGTGTAGGCGATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 658 |
| LEAP-Seq percent confirming: | 83.6501 |
| LEAP-Seq n confirming: | 5766 |
| LEAP-Seq n nonconfirming: | 1127 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTTGGTTTCCACAAGAA |
| Suggested primer 2: | GCGTGTGTGTATGTGTGTGC |