| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235386 |
| Chromosome: | chromosome 3 |
| Location: | 7815743 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202950 | CRX1,CRX,TRX14 | Calredoxin 1; (1 of 1) PTHR10891//PTHR10891:SF569 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACCCACGTCCACTGGTCCACTTCCTCA |
| Internal bar code: | TGGGACTGCGCTGGCTGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1054 |
| LEAP-Seq percent confirming: | 97.7855 |
| LEAP-Seq n confirming: | 11481 |
| LEAP-Seq n nonconfirming: | 260 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGGTTTACGGAATGTCT |
| Suggested primer 2: | CGCAGTTCAACTTTATCGCA |