| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235387 |
| Chromosome: | chromosome_17 |
| Location: | 1143419 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g704300 | FAP47 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCCGGTCCAACCAGATCAAGGCGCCGTAC |
| Internal bar code: | CCCTCCTGACGGCTGGTGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 197 |
| LEAP-Seq percent confirming: | 99.5832 |
| LEAP-Seq n confirming: | 2389 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTCCAGGTTGTCGTAGC |
| Suggested primer 2: | CTGGATACGGGACAAGGAAA |