| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235426 |
| Chromosome: | chromosome 3 |
| Location: | 6209553 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192400 | (1 of 1) IPR013769 - Band 3 cytoplasmic domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAGCTTGGCCGGGCCGGCAGGCCTACG |
| Internal bar code: | GCACGTGGACCCGGTGGACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 95.93 |
| LEAP-Seq n confirming: | 7401 |
| LEAP-Seq n nonconfirming: | 314 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACGGAAGGTAGCTTGA |
| Suggested primer 2: | GTGTTACACGGCATGACTGG |