Insertion junction: LMJ.RY0402.235449_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.235449
Chromosome:chromosome 9
Location:2603360
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g390350 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCTTGGCGGGGGTGCGCGTGTCTGCGGC
Internal bar code:GTGAGAACTCCGACACACCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:931
LEAP-Seq percent confirming:99.5909
LEAP-Seq n confirming:26047
LEAP-Seq n nonconfirming:107
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CATTCACACCAGGGTCTGTG
Suggested primer 2:ACTCGCCAGGGTTACATGAC