| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235451 |
| Chromosome: | chromosome 6 |
| Location: | 3999359 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278199 | (1 of 4) 1.13.11.75 - All-trans-8'-apo-beta-carotenal 15,15'-oxygenase / 8'-apo-beta-carotenal 15,15'-oxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACATACTCGACAAGAGCGGCCAAGCAC |
| Internal bar code: | TTGTCGGGCCTGGGACGGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 511 |
| LEAP-Seq percent confirming: | 96.5704 |
| LEAP-Seq n confirming: | 535 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTTGAACGCGTATCCTG |
| Suggested primer 2: | CCGAAGGCTCTAACTGAACG |