| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235494 |
| Chromosome: | chromosome 4 |
| Location: | 1555187 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213652 | TERT1,TERT,CrTERT | Telomerase reverse transcriptase; (1 of 1) K11126 - telomerase reverse transcriptase (TERT, EST2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCACAAGGGGTTATGGGGAGGGTGGCGT |
| Internal bar code: | AGGCCCCATTTAGTCAAGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 99.6353 |
| LEAP-Seq n confirming: | 2732 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTAAAACCATGCACACCT |
| Suggested primer 2: | ACATGTAGGAGATGGCGGAC |