| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235497 |
| Chromosome: | chromosome 1 |
| Location: | 3488094 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022150 | KCN3 | Voltage-gated K+ channel; (1 of 1) PTHR11537//PTHR11537:SF158 - VOLTAGE-GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTGCCAACATGCAAACCCAGACCTATG |
| Internal bar code: | ACCACGATACATGTCGCCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 99.3567 |
| LEAP-Seq n confirming: | 18997 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGCATGGTGGTAATGAA |
| Suggested primer 2: | TCGGTGAATCTCTTGTGCTG |