| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235520 |
| Chromosome: | chromosome 12 |
| Location: | 2154579 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508650 | (1 of 2) PF02010 - REJ domain (REJ) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAGCGGGCGCGGGCTGCGGCAGCATGG |
| Internal bar code: | TGAGCGTTGGATAGTACCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1144 |
| LEAP-Seq percent confirming: | 99.6224 |
| LEAP-Seq n confirming: | 20841 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGAGGATTCTGAACAGC |
| Suggested primer 2: | ATAGGCGAGTGAGCTGGTGT |