| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235522 |
| Chromosome: | chromosome 3 |
| Location: | 4593573 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177007 | (1 of 1) PTHR12480:SF6 - JMJC DOMAIN-CONTAINING PROTEIN 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAATCCTTGCGTAATTACTACCCGTATAA |
| Internal bar code: | ATTGAAATTCTTCCTTTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 98.7541 |
| LEAP-Seq n confirming: | 5390 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGACGGAGAAGGAGGAG |
| Suggested primer 2: | CGCAGGCCGTTATACATTTT |