Insertion junction: LMJ.RY0402.235522_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.235522
Chromosome:chromosome 9
Location:7865940
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCAGTCGCAACCGGTCCCACCGCACCCCG
Internal bar code:GTCCTTCCTGTACCCGAGGCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:629
LEAP-Seq percent confirming:99.4288
LEAP-Seq n confirming:4526
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:TCTAGACAACGTGGCAGCAC
Suggested primer 2:GGTCCAACGCCTGTAAGTGT