Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.235522 |
Chromosome: | chromosome 9 |
Location: | 7865940 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGTCGCAACCGGTCCCACCGCACCCCG |
Internal bar code: | GTCCTTCCTGTACCCGAGGCGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 629 |
LEAP-Seq percent confirming: | 99.4288 |
LEAP-Seq n confirming: | 4526 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTAGACAACGTGGCAGCAC |
Suggested primer 2: | GGTCCAACGCCTGTAAGTGT |