| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235527 |
| Chromosome: | chromosome 14 |
| Location: | 3329396 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629920 | AMT1B,AMT2 | (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP); Ammonium transporter (AMT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTGCTAAGGCGTCACGAAGAGGCGGTG |
| Internal bar code: | ACACGCGCGCCCCGGGGTACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1137 |
| LEAP-Seq percent confirming: | 99.2894 |
| LEAP-Seq n confirming: | 7405 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGGTCGACACTATTGGG |
| Suggested primer 2: | GACAACCGGCTCTTGACCTA |