| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235535 |
| Chromosome: | chromosome 2 |
| Location: | 3329370 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095090 | (1 of 25) IPR013216//IPR029063 - Methyltransferase type 11 // S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTCAAGGGGCAGGTCGGCAGAGTCCCT |
| Internal bar code: | GGCTATAGGTACTACTCGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 641 |
| LEAP-Seq percent confirming: | 74.7303 |
| LEAP-Seq n confirming: | 4711 |
| LEAP-Seq n nonconfirming: | 1593 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCATTCACTCGCAAGCAC |
| Suggested primer 2: | AGGACGGACTGCAATCAAAC |