| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235542 |
| Chromosome: | chromosome 12 |
| Location: | 6823705 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561350 | (1 of 1) PTHR13244//PTHR13244:SF8 - ZINC FINGER MYND DOMAIN CONTAINING PROTEIN 10 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTATACAGAACACCAGAAGGCAAGCATG |
| Internal bar code: | CTCGGACTCGGACGCCATACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1180 |
| LEAP-Seq percent confirming: | 97.5995 |
| LEAP-Seq n confirming: | 4391 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATCCACCTGCCGTACAAG |
| Suggested primer 2: | CACAGAAGCGAAGGCATGTA |