| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235571 |
| Chromosome: | chromosome 7 |
| Location: | 3700862 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337582 | (1 of 1) PF03448 - MgtE intracellular N domain (MgtE_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTACAACCTTCTCCCCCCCTGCCTTCCC |
| Internal bar code: | CGAGGGGTGACGCGTTCCGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 99.6395 |
| LEAP-Seq n confirming: | 12713 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTGTAGATACCAGCCCA |
| Suggested primer 2: | AGGTCCTCTGTGTGTTTGGG |