| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235584 |
| Chromosome: | chromosome 7 |
| Location: | 3558674 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336350 | (1 of 1) PTHR24012//PTHR24012:SF432 - FAMILY NOT NAMED // HEPHAESTUS | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATGGGGAGGTCTGCGTGGTACCGCAAG |
| Internal bar code: | TAGTGCACCCTACGAATTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1177 |
| LEAP-Seq percent confirming: | 99.5617 |
| LEAP-Seq n confirming: | 1590 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCAAAGTAATCAAGGCG |
| Suggested primer 2: | GTGTGTGTTGTGAAGGGCAC |