| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235588 |
| Chromosome: | chromosome 5 |
| Location: | 1865687 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241751 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATGTGCACCGTCGTCAAAAGACAGCGC |
| Internal bar code: | GGTCCTGGTGGTGCCCTTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1168 |
| LEAP-Seq percent confirming: | 99.2522 |
| LEAP-Seq n confirming: | 20042 |
| LEAP-Seq n nonconfirming: | 151 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTACAAGTGGTCAAGGC |
| Suggested primer 2: | CTGACACGGCTGATGACTGT |