Insertion junction: LMJ.RY0402.235609_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.235609
Chromosome:chromosome 2
Location:2434234
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g091500 (1 of 1) PTHR13595:SF1 - ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 6-INTERACTING PROTEIN 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCCAAACCAGGGCCACACCTGCATCATC
Internal bar code:ATCCACGATGTGTTGGGGGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:664
LEAP-Seq percent confirming:73.1567
LEAP-Seq n confirming:1657
LEAP-Seq n nonconfirming:608
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCCCATGAATCTGTAATG
Suggested primer 2:ATTTAACTTCCGCCACAACG