| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235611 |
| Chromosome: | chromosome 17 |
| Location: | 6073079 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741250 | PHR1,PHR6 | (1 of 1) 2.5.1.77 - 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase; 7%252C8-didemethyl-8-hydroxy-5-deazariboflavin synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTCTGTCATGCACAGCTGCCCTGACTT |
| Internal bar code: | AGGGGCTGAAATGACTCACAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 99.6205 |
| LEAP-Seq n confirming: | 525 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCATTTTGGTGTGTGTG |
| Suggested primer 2: | GGATGTTAGGGATGTGTGGG |