| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235635 |
| Chromosome: | chromosome 6 |
| Location: | 2931933 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272701 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACAGAAATTGGCAAAACGTCACCGTAGC |
| Internal bar code: | CCTTCGGAGTCGCCCGAATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 98.4241 |
| LEAP-Seq n confirming: | 2748 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAGCATGACAGTGCGTT |
| Suggested primer 2: | TAGACAGACAAACCGCAACG |