| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235636 |
| Chromosome: | chromosome 11 |
| Location: | 1202242 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467693 | (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTCACGCTAGACACATTCCTCATCGGCA |
| Internal bar code: | GATCTCCCGCGGCGGCGGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 190 |
| LEAP-Seq percent confirming: | 93.2961 |
| LEAP-Seq n confirming: | 334 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACACACATTGCAACCTCC |
| Suggested primer 2: | GACCGATGCCCTCTAACAAA |