| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235643 |
| Chromosome: | chromosome 6 |
| Location: | 8979914 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311600 | RFK3 | Riboflavin kinase; (1 of 1) PTHR22749//PTHR22749:SF1 - RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATGGGTGTGGCGCAATTTAATTTTTGTG |
| Internal bar code: | AAAATAGTCGACCGGTCTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 98.4818 |
| LEAP-Seq n confirming: | 35613 |
| LEAP-Seq n nonconfirming: | 549 |
| LEAP-Seq n unique pos: | 115 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGTTTCTTCCCGTGTT |
| Suggested primer 2: | ATCCACAAGACGGTGAGAGG |