| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235645 |
| Chromosome: | chromosome_17 |
| Location: | 3434752 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g724500 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTGGACAGGGCGTGGTCATGTCGACGACG |
| Internal bar code: | CCGTTCGTAGCTTCGTGCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 96.9883 |
| LEAP-Seq n confirming: | 1900 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGAGCACCAGTAGCGTCT |
| Suggested primer 2: | GTCAAACTTCGCACCACCTT |