| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235646 |
| Chromosome: | chromosome 2 |
| Location: | 6275727 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g114500 | (1 of 1) 3.1.3.75 - Phosphoethanolamine/phosphocholine phosphatase / PHOSPHO1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATCAAGGCAAGTGTGACAAAGACGAGGA |
| Internal bar code: | CACGCGAGCGTGGACTGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 99.2095 |
| LEAP-Seq n confirming: | 2259 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTTCATCAACCACATGC |
| Suggested primer 2: | CCTTGTGATAGACCGGGAAA |