Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.235676 |
Chromosome: | chromosome 6 |
Location: | 4950214 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g279700 | (1 of 1) PTHR15535:SF22 - PROTEIN RLIB | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATTCCCACACGGGCTTCCCATCGTTGC |
Internal bar code: | GGCAGTTACTCGACTAGACGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 725 |
LEAP-Seq percent confirming: | 97.3305 |
LEAP-Seq n confirming: | 2297 |
LEAP-Seq n nonconfirming: | 63 |
LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCGTCTGACAGCTACATGA |
Suggested primer 2: | AGACTCACGTGGGGTCAAAC |