Insertion junction: LMJ.RY0402.235678_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.235678
Chromosome:chromosome 13
Location:5171511
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g607800 (1 of 29) IPR013320 - Concanavalin A-like lectin/glucanase domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCAGCGTTAGCCTCCAGGGGCAATGCC
Internal bar code:CGGTTGCTAGTCGCGCTCGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:379
LEAP-Seq percent confirming:99.1711
LEAP-Seq n confirming:5025
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGACTACGTCCACCTCGC
Suggested primer 2:CGTGCCACTTCTATGCTTCA