| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.235707 |
| Chromosome: | chromosome_17 |
| Location: | 2080692 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g712100 | PNO2,MDAR1 | Pyridine nucleotide-disulphide oxidoreductase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACAGCATCTTCCCCAGCACCTCCCTCGAA |
| Internal bar code: | GACCCGCTTTTCGGCATTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 202 |
| LEAP-Seq percent confirming: | 46.1538 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAGATGGAGATGCTGCT |
| Suggested primer 2: | TCAACTCAGACTCTGCCCCT |