| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235708 |
| Chromosome: | chromosome 12 |
| Location: | 133849 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTGGAAGCAACAGTGAGGGCGGCTGTT |
| Internal bar code: | CGGTATAAGTGCACCGGGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 98.5 |
| LEAP-Seq n confirming: | 2364 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGAGAGAACGTGCAAGG |
| Suggested primer 2: | CAAGAACCCCGTGCTGTAAT |