| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235712 |
| Chromosome: | chromosome_17 |
| Location: | 5146083 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g735350 | FAP164 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTCATGAAGGCTGTTCGTTTCGCAGGCTC |
| Internal bar code: | GTTCACATAGGGAAGGTTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 99.4516 |
| LEAP-Seq n confirming: | 61838 |
| LEAP-Seq n nonconfirming: | 341 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTAACACAGTCCCAAGA |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |