Insertion junction: LMJ.RY0402.235712_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.235712
Chromosome:chromosome 17
Location:5146083
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g735350 FAP164 (1 of 1) PTHR24198//PTHR24198:SF57 - ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN // SUBFAMILY NOT NAMED; Ankyrin Repeat Flagellar Associated Protein 164 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCATGAAGGCTGTTCGTTTCGCAGGCTC
Internal bar code:GTTCACATAGGGAAGGTTGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:316
LEAP-Seq percent confirming:99.4516
LEAP-Seq n confirming:61838
LEAP-Seq n nonconfirming:341
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTAACACAGTCCCAAGA
Suggested primer 2:CCAGCCCAAACTAAACCAAA