| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235735 |
| Chromosome: | chromosome 6 |
| Location: | 3516333 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278107 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAGGCAGCCTCGATGTGGACGGCAAGG |
| Internal bar code: | GCAGATGGGGTTTTTGACCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 67.6692 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGTACAGTCGCGGAGAT |
| Suggested primer 2: | TTGTCATCAAGTCCACCAGC |