| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235735 |
| Chromosome: | chromosome 6 |
| Location: | 5977768 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289000 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAAAGCCAGGGCGGAGTGTACAGTTTGT |
| Internal bar code: | TAAACGTTTTGTGTCGGAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 99.4913 |
| LEAP-Seq n confirming: | 1369 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAGACGTCCCCGATACG |
| Suggested primer 2: | AGGCCAGACCACATAACCAG |