| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235758 |
| Chromosome: | chromosome 9 |
| Location: | 2663273 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389950 | OAT1 | O-acetyltransferase-related protein; (1 of 1) 2.3.1.45 - N-acetylneuraminate 7-O(or 9-O)-acetyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAAAACCGTGGCAGGCATCCCTCACTCC |
| Internal bar code: | AAAATCTCTTGCATTTGGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 977 |
| LEAP-Seq percent confirming: | 99.503 |
| LEAP-Seq n confirming: | 15215 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAAAACCGGGGACTTCTG |
| Suggested primer 2: | AGGTTGGATTGCACGTAAGG |