| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235770 |
| Chromosome: | chromosome 16 |
| Location: | 4175959 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668800 | MTA4 | Predicted protein; (1 of 2) PTHR24032:SF25 - SPORE COAT PROTEIN SP85 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTATGCAACCGAAACATGTGTGCACAT |
| Internal bar code: | GCTTTATGAAGATAGTTGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1030 |
| LEAP-Seq percent confirming: | 99.411 |
| LEAP-Seq n confirming: | 12996 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGTGTGGAGGACCAGTG |
| Suggested primer 2: | ATCCACTTGAAACTCCCACG |