| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.235776 |
| Chromosome: | chromosome 6 |
| Location: | 2657009 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270050 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCCTGTCGACCGCTTCTGCAGGCCGC |
| Internal bar code: | GATGACTTACACAGTGGTAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 99.5741 |
| LEAP-Seq n confirming: | 2572 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAACTGCGAGGACTGAAA |
| Suggested primer 2: | CGGCTGATCTGATGGATTTT |