Insertion junction: LMJ.RY0402.235781_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.235781
Chromosome:chromosome 2
Location:2783881
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g094150 HIK,HIK2,HKR2 (1 of 1) K12130 - pseudo-response regulator 5 (PRR5); Response regulator of two-component system 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTGCATACTGCCGCCTTCCCGTCACGCT
Internal bar code:GTGTGCGGCGGTGCCCCGCCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:133
LEAP-Seq percent confirming:7.69231
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCATACATTTCCGAGTCC
Suggested primer 2:GGAGACTGGTTAGTTGCCCA