| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.235781 |
| Chromosome: | chromosome 2 |
| Location: | 2784251 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094150 | HIK,HIK2,HKR2 | (1 of 1) K12130 - pseudo-response regulator 5 (PRR5); Response regulator of two-component system | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCGTTCACCCTCTGCTTGATGTTGCT |
| Internal bar code: | AGGTGTACGTTTGCTGTCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 0.0143123 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 6986 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCGTCTGCGTCTCTTTC |
| Suggested primer 2: | CAGCCACCTCCTTGTTTGTT |