| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.235784 |
| Chromosome: | chromosome_11 |
| Location: | 1540295 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g467753 | HMO2 | Heme oxygenase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACTGGGAGGTCCGCTGGGGAGCCTGGAAG |
| Internal bar code: | TTTGTTACTAACAGGAAACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 39.0548 |
| LEAP-Seq n confirming: | 876 |
| LEAP-Seq n nonconfirming: | 1367 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAAACCTCCGAGACATGC |
| Suggested primer 2: | GTACTGATGGGGTCTTCGGA |